Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules.
Synonyms: AGO2 Antibody - Monoclonal Anti-AGO2 antibody produced in rat; Ago2 Antibody; Anti-Argonaute 2; Anti-EIF2C2 eukaryotic translation initiation factor 2C, 2; Anti-Q10
Storage: -20C
Application: Monoclonal Anti-AGO2 antibody produced in rat has been used in:
•immunofluorescent localization
•western blot analysis
•immunoprecipitation.
Biochem Physiol Actions: Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients.
Other Notes: Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.