Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.
MDL Number: MFCD00145889
Storage: -20C
Application: Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
•two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
•immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
•immunoblotting
Biochem Physiol Actions: In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.