The MISSION(R) TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION(R) shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/µl.
Synonyms: MISSION(R) Control Vectors; negative control; negative shRNA control; non-target control; non-target shRNA; non-target shRNA control; shRNA control
MDL Number: MFCD07785395
Storage: -20C
Application: To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information: Use of this product is subject to one or more license agreements. For details, please see issionlicense">http://sigmaaldrich.com/missionlicense.