Sigma shRNA Human Kinase Pooled Lentiviral LibrarySigma-Aldrich has a long-standing expertise in pooled lentiviral screening spanning whole genome and custom pool offerings. Due to the popular demand of the kinase gene panel, we are proud to offer an off-the-shelf, ready-to-use shRNA kinase pool optimized to meet researchers' needs. This pooled library targets the human kinome, a collection of approximately 745 genes commonly associated with development and disease, with an average of 12.6 shRNA clones per unique kinase gene. More importantly, this shRNA pool contains important enrichment, depletion, and non-target controls so you can be confident in your results, and reduce the likelihood of false positives or negatives. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells, allowing researchers to functionally screen hundreds of kinase genes for a fraction of the time and cost of screening arrayed clones. Whether you are interested in small molecule screening or in defining developmental signaling pathways, Sigma's shRNA human kinase pooled lentiviral library offers researchers a high throughput solution to uncover novel kinase targets, especially when essential and lethal genes are important.Differences "between hit genes called by CRISPRi and RNAi are not necessarily an indication of technical shortcomings of either method, but can also guide new biological discoveries. The difference [lies] in the mode of action of CRISPRi and RNAi: Whereas CRISPRi targets entire transcription units, including alternative splice isoforms and embedded ncRNAs, RNAi targets mature transcripts and thus differentiates between different products processed from the same primary transcript. Parallel CRISPRi and RNAi screens can reveal novel transcript functions in cases where CRISPRi and RNAi phenotypes for reagents designed to target the same gene product differ." From Kampmann et. al. PNAS (2014) 112(26): June, 2015.Please also see our Human Kinase Lentiviral CRISPR Pool for more information on how to complement your RNAi screen(s).
Storage: -70C
Application: Functional Genomics/Screening/Target Validation
Legal Information: MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes: This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.