This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.The MISSION® TRC2 Control Vector pLKO-puro Non-Target shRNA is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE.Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 Non-Target shRNA Control Vector is provided as 10 mug of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/mul.
Synonyms: MISSION® Control Vectors
MDL Number: MFCD07785395
Storage: -20C
Application: To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information: Use of this product is subject to one or more license agreements. For details, please see issionlicense">http://sigmaaldrich.com/missionlicense.