Synonyms: MISSION(R) siRNA Transfection Reagent
Storage: 2-8C
UNSPSC Code: 12352200
General description: The MISSION(R) siRNA Transfection Reagent is for the transfection of siRNA to achieve >=90% silencing efficiency for transient knockdown of eukaryotic gene expression. Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Other Notes: Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 muL of the MISSION(R) siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
RIDADR: UN1170 - class 3 - PG 3 - Ethanol, solution
WGK Germany: 3
Flash Point(F): 120.2 F
Flash Point(C): 49 C
R Codes: 10
Symbol: GHS02
Signal Word: Warning
Hazard Statements: H226