The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The pLKO.1-puro Control Vector is provided as 10 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/µl.
Synonyms: MISSION® Control Vectors
MDL Number: MFCD07785395
Storage: -20C
Application: To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information: Use of this product is subject to one or more license agreements. For details, please see issionlicense">http://sigmaaldrich.com/missionlicense.