MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1a belongs to the CCL chemokine family, and shares 68% homology with MIP-1ß. The mature form of MIP-1a contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1ain vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1ß, Interferon-?, and lipoteichoic acid from gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1a. MIP-1a augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. Additionally, in wounds, MIP-1achemoattracts macrophages in order to accelerate the tissue repair process.
Recombinant Mouse MIP-1 alpha/CCL3 (rmMIP-1a) produced in HEK 293cells is a single polypeptide chain containing 69 amino acids. A fully biologically active molecule, rmMIP-1ahas a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.