The synthesis and release of Triiodothyronine (T3) and thyroxine (T4) are regulated by Thyroid-Stimulating Hormone (TSH), which is secreted by the anterior pituitary gland. These thyroid hormones play critical roles in the regulation of energy balance, metabolism of carbohydrates, fatty acids and proteins. Thyroid hormones are critical to the development of the central nervous and skeletal systems. The simultaneous monitoring of all three hormones can be of great value when researching disease models and therapeutic response. MILLIPLEX(R) Rat Thyroid Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: T3 (competitive assay), T4 (competitive assay), and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Endocrine
Application:
•Analytes: T3, T4, TSH
•NOTE: T3 and T4 are competitive immunoassays
•Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants or lysates
•Recommended Sample Dilution: 25 muL per well 1:6 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
•Assay Run Time: Overnight (16-18 hours) at 2-8C
•Research Category: Endocrine
•Research Category: Metabolism
Legal Information: xMAP is a registered trademark of Luminex Corp
Other Notes: Please contact Technical Service for linearity of dilution.