Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems. MILLIPLEX(R) Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism
Application:
•Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
•Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
•NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
•Recommended Sample Dilution: 25 muL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
•Assay Run Time: Overnight (16-18 hours) at 2-8C or 2 hours at room temperature (20-25C)
•Research Category: Bone
•Research Subcategory: Metabolism
Legal Information: xMAP is a registered trademark of Luminex Corp
Other Notes: Please contact Technical Service for linearity of dilution.