Cardiovascular disease, particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO, 2010). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk factors. MILLIPLEX(R) Human Cardiovascular Disease (CVD) Panel 3 (Acute Phase) is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma or tissue/cell lysate and culture supernatant samples: Adipsin, alpha-1-Acid-Glycoprotein (AGP), alpha-2-Macroglobulin (A2M), C Reactive Protein (CRP), Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P (SAP), Haptoglobin, and Platelet Factor-4 (PF4). (Please note: Fibrinogen is not detectable in serum.) This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cardiovascular
Application:
•Analytes: alpha-1 Acid Glycoprotein (AGP), alpha-2-Macroglobulin (A2M), Adipsin (Factor D), C Reactive Protein (CRP), Fetuin A, Fibrinogen, Haptoglobin, sL-Selectin, Platelet Factor-4 (PF4/CXCL4), Serum Amyloid P (SAP)
•Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants and lysates
•Recommended Sample Dilution: 25 muL per well of 1:40,000 diluted serum or plasma; tissue/cell culture samples may require dilution in appropriate control medium
•NOTE: Fibrinogen is not detectable in serum
•Assay Run Time: Overnight (16-18 hours) at 2-8C or 2 hours at room temperature (20-25 C)
•Research Category: Cardiovascular Disease
•Research Subcategory: Metabolic Disorders
Legal Information: xMAP is a registered trademark of Luminex Corp
Other Notes: Sensitivity: See kit protocol for individual analyte sensitivity.