Universal method to isolate and detect microorganisms and host cells simultaneously
For use with samples collected using a swab - fresh, frozen or preserved in Norgen's Swab Collection and DNA Preservation System or Norgen's Fecal Swab Collection and Preservation System
Rapid and convenient spin-column format
Remove all PCR inhibitors from DNA samples
Isolate high quality total DNA for PCR and NGS applications (Microbiome/Metagenomic sequencing)
Optimized to isolate from the full preservative volume of the Swab Collection and DNA Preservation System for maximum yield
Norgen's Microbiome DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from a variety of Microbiome samples collected using a swab. This kit can be used for fresh (dry transport) swab or any swab preserved using Norgen's Swab Collection and DNA Preservation Kit or any other of Norgen's Preservation Systems. This universal protocol conveniently allows for the isolation of total genomic DNA from both host and microbial cells found in the swab sample simultaneously. The kit removes all traces of PCR impurities using the provided combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR or metagenomics using NGS.