Synonyms: Methotrexate hydrate; 4-Amino-10-methylfolic acid hydrate; L-4-Amino-N10-methylpteroylglutamic acid hydrate; L-Amethopterin hydrate; Antifolan hydrate; MTX hydrate; Methylaminopterin hydrate
Formula: C20H22N8O5 · xH2O
Formula Weight: 454.44 (anhydrous basis)
CAS No.: 133073-73-1
Storage Temperature: −20°C
Application: Potent inhibitor of dihydrofolate reductase1 and agent for antitumor studies.2,3 Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Biochem/physiol Actions: Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.
Gene Information: human ... DHFRP1(573971), FPGS, SLC19A1
Storage Temp.: −20°C
Suitability: meets USP testing specifications