Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets:
•It deletes the first 11 amino acids in the Helix 1 portion (referred to as "H0.5" in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1).
•It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
Synonyms: Membrane Scaffold Protein 1E3D1; MSP1E3D1
Purity: >=90% (SDS-GE)
Storage: -20C
Application: The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase.
Biochem Physiol Actions: Generates Nanodiscs ~12.9 nm in diameter
Legal Information: Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
•7,691,414 Membrane scaffold proteins
•7,662,410 Membrane scaffold proteins and embedded membrane proteins
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•7,048,949 Membrane scaffold proteins