Synonyms: Mca-APK(Dnp) TFA salt (Mca = 7-Methoxycoumarin-4-yl; Dnp = 2,4-dinitrophenyl); Mca-Ala-Pro-Lys(Dnp) TFA salt; Mca-Ala-Pro-Lys(Dnp)-OH TFA salt
Molecular Formula: C32H36N6O12
Molecular Weight: 696.66
Linear Structural Formula: C32H36N6O12
Purity: >=97% (HPLC)
Storage: -20C
Biochem Physiol Actions: Mca-APK(Dnp)-OH is a widely used fluorescent substrate for angiotensin-converting enzyme 2 (ACE2 cleaves between P & K). Proteolytic cleavage separates 7-methoxycoumarin-4-acetyl (Mca) from the fluorescence-quenching group 2,4-dinitrophenyl (Dnp), resulting in increased Mca fluorescence (Ex: 320-328 nm, Em: 405-420 nm). The fluorescence increase over background upon Mca-APK(Dnp) cleavage is more than 14-fold higher than that of Mca-YVADAPK(Dnp), while only Mca-YVADAPK(Dnp), but not Mca-APK(Dnp), can also be used for assessing caspase-1 (ICE) activity.