M9, Minimal Salts, 5X powder, minimal microbial growth medium

Components
33.9g/L Na₂HPO₄
15g/L KH₂PO₄
5g/L NH₄Cl
2.5g/L NaCl

Features and Benefits
M9 powder provides:

• Standard formulation
• A budget-friendly alternative to liquid

General Description
M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

Packaging
1 kg in poly bottle

Reconstitution
Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).