Loeffler Medium Base is recommended for cultivation of Corynebacterium diphtheriae from clinical specimens and in pure cultures, detection of chromogenesis, proteolysis and the production of ascospores. Addition of sterile Horse Serum (RM1239) enriches the medium. It is recommended to be used in parallel with Serum Tellurite agar for selective isolation of Corynebacteria. The high serum content favors determining proteolytic activity of organisms. It can also used for demonstration of pigmentation and ascospores.
Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis
- Appearance: Cream to yellow homogeneous free flowing powder
- Color and Clarity of prepared medium Basal: Light amber colored clear to slightly opalescent solution. After addition of horse serum: Off-white colored opaque solution
- Reaction: Reaction of 3.52% w/v aqueous solution of base at 25°C. pH : 7.3±0.2
- Cultural Response: Cultural characteristics observed with added 750ml horse serum, after an incubation at 35-37°C for 24-48 hours