Synonym: Linoleate:oxygen oxidoreductase, Lipoxygenase
CAS Number: 9029-60-1
EC Number: 232-853-1
Enzyme Commission (EC) Number: 1.13.11.12
MDL Number: MFCD00131527
Analysis Note
Protein determined by biuret.
Application
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Preparation Note
Prepared by affinity chromatography.
Unit Definition
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Physical Form
Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0
Type: Type V
Form: ammonium sulfate suspension
Mol. Wt.: mol wt 108 kDa (two 54 kDa subunits)
Storage Temp.: 2-8°C