Synonym: PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
Enzyme Commission (EC) Number: 3.1.1.3
MDL number: MFCD00131509
Analysis Note
Protein determined by biuret.
Quality
Crude (Steapsin). Contains amylase and protease activity.
Unit Definition
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Application
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions.1,2,3 They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).4
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.