Lipases are a subclass of the esterases. It catalyzes the hydrolysis of emulsified esters of glycerol and long chain fatty acids.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Key Applications: Resolution of chiral compounds, Transesterification production of biodiesel.
Application Areas: Molecular biology
Product Type: Proteins, Enzymes & Peptides
Extinction Coefficient (E1%): E1%280 = 13.3(Lit.)
Presentation: Cream Colored Powder
Format: Powder
Isoelectric point (pI): Lipase A = 4.9 and Lipase B = 5.0 (Lit.)
NOTES: Activators: Ca2+ is required for activity [Sr2+ and Mg2+ are less effective activators. Inhibitors: Versene, Zn2+, Cu2+, Hg2+, iodine, PCMB. DFP does not inhibit. Stabilizers: DFP may be used to stabilize impure preparations containing proteinases in solutions.
Solubility: Partly soluble in water and insoluble in alcohol.
Storage & Handling: -20°C