Lentivirus vector is derived from human immunodeficiency virus-1 (HIV-1), which has been widely used to transfer gene(s) of interest into dividing and non-dividing cells. The lentiviral titer, as assessed by the p24 protein content, is an essential characterization procedure for routine quality control and lot release, as well as for purification process optimization. However, HEK-293T cells will release free p24 protein into the supernatant during transfection, and this part of p24 would account for a large percentage of the total p24. The traditional p24 ELISA kit detects both lentivirus associated p24 and free p24, which will overestimate the titer of the lentivirus.
GenScript’s Lentivirus Titer p24 ELISA Kit Pro(Cat. No. L00974) is a Sandwich ELISA designed for quantitatively measuring the physical titer of any HIV-1 based lentivirus in cell culture supernatant. After removing free p24 (patented technology), the amount of lentivirus-associated p24 can be measured by the p24 ELISA kit. The kit has the same linear range, detection capability, and precision with Lentivirus Titer p24 ELISA Kit (Cat. No. L00938) in the detection of p24 concentration. This kit makes lentivirus titration easy, accurate and reproducible.