Laminin from human placenta, liquid, BioReagent, suitable for cell culture

CAS Number: 114956-81-9

MDL No.: MFCD00081739

Storage: -70C

UNSPSC Code: 12352200

Application: Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 mug/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions: Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components: Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Preparation Note: This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 mum filtered. Thaw this solution slowly before use at 2-8C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8C.