Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma's RPTEC's exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and alpha-GST. Sigma's RPTEC's maintain typical PT functions including albumin reabsorption, gamma-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.
Storage: -196C
Application: Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture. RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors. RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate(R) in both toxicity and barrier integrity assays ( Z742750).RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01).
Legal Information: These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.