Synonym: Kanamycin A, Kanamycin acid sulfate
CAS Number 64013-70-3
MDL number MFCD00143916
Preparation Note
Kanamycin acid sulfate is prepared by solubilizing kanamycin sulfate in dilute sulfuric acid and drying. The sulfate content is approximately 25%.
Application
Aminoglycoside-antibiotic with broad antibacterial spectrum, (including mycobacteria, many gram-positive and most gram-negative organisms); Additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance); on a kanamycin-medium1,2. Kanamycin has been used for electrophysiological recordings of outer hair cells and inner hair cells3. It is used to inhibit shoot regeneration from Siberian Elmleaf explants4.
Biochem/physiol Actions
Kanamycin inhibits shoot regeneration of Siberian Elm leaf explants4. Kanamycin is an aminoglycoside antibiotic. Mode of Action: Binds to 70S ribosomal subunit; inhibits translocation; elicits miscoding. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasm.