J'sphere offers a choice from three kinds of ODS made from the same silica gel base, each with different ligand coverage. Differences in ligand coverage considerably affect the hydrophobic retention behavior of solutes, as well as the separation behavior resulting from solute functional groups or tertiary structure. J'sphere is useful for the optimization of separation conditions, since there is almost no need to consider interactions other than hydrophobic and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).
Application: For Complexing Agents, Pharmaceuticals
- Useful for method development
- High theoretical plate number
- ODS-L80, with 9% carbon offers retention similar to C8, but with unique selectivity imparted by its high hydrogen-bonding capacity