J'sphere offers a choice from three kinds of ODS made from the same silica gel base, each with different ligand coverage. Differences in ligand coverage considerably affect the hydrophobic retention behavior of solutes, as well as the separation behavior resulting from solute functional groups or tertiary structure. J'sphere is useful for the optimization of separation conditions, since there is almost no need to consider interactions other than hydrophobic and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).
Application: For Complexing Agents, Pharmaceuticals
- Useful for method development
- High theoretical plate number
- ODS-H80, with 22% carbon is the most hydrophobic and hydrolytically stable. It resists attack by both high- and low-pH solvents and has proven stable at pH's 1.1 to 9.3 for over 500 hours!