Each kit contains 10 packs of 2 cassettes each: one cassette for detecting IgG1, IgG2a and IgG2b; the other cassette for detecting IgG3, IgA and IgM. When a properly diluted sample containing a specific isotype antibody is added to the sample well, a specific class and subclass soluble complex is formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and anti-class-specific antibody impregnated membrane. A control line will appear on the membrane in the region marked ''C'' on the cassette indicating a successful run (positive internal control). Typically when antibodies are applied at a concentration of 10 ng/mL the best results are obtained by reading the cassette 5 to 10 minutes after application. Results should NOT be read after 10 minutes.