Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside, iptg
CAS Number: 367-93-1
Empirical Formula (Hill Notation): C9H18O5S
Molecular Weight: 238.30
Beilstein Registry Number: 4631
MDL Number: MFCD00063273
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Very effective inducer of the lac operon for β-galactosidase — binds and inactivates the lac repressor.
General Description
Chemical analog of galactose that cannot be cleaved by β-galactosidase.
Other Notes
Non-metabolizable galactose analog.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:
- amount of template DNA
- purity of template DNA
- conformation of template DNA
- average size of labeled fragment
Preparation Note
Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.
Specifications
Assay Time: 50 minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Mol. Wt.: mol wt 238.3
Packaging: pkg of 1 g (10724815001); pkg of 5 g (11411446001)
Mfr. No.: Roche
Shipped In: wet ice
Storage Temp.: 2-8°C