Insulin is the primary polypeptide hormone responsible for controlling the cellular uptake, utilization and storage of glucose, amino acids and fatty acids while inhibiting the breakdown of glycogen, protein and fat. It is produced by pancreatic beta cells.
Methods for immobilizing insulin on polystyrene dishes have been reported for applications involving protein-free cell cultures.
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Specific Activity: ~28 IU/mg
Product Type: Proteins, Enzymes & Peptides
Extinction Coefficient (E1%): EM (278 nm): 6080 (33 mM phosphate, pH 7.0, bovine and porcine) (Lit.)
Presentation: Powder
Format: Powder
Isoelectric point (pI): ~5.3 (for the native protein)(Lit.)
NOTES: Zinc Content: 0.25 - 1.1%
Solubility: Insulin is not soluble at neutral pH. It can be solubilized (1 - 10 mg/mL) in dilute acetic acid or dilute hydrochloric acid, pH 2-3.
Storage & Handling: Store at -20°C.