Synonym: Glyoxaline solution, Imidazole solution
CAS Number: 288-32-4
Empirical Formula (Hill Notation): C3H4N2
Molecular Weight: 68.08
Application
Imidazole can be used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.
Product line: BioUltra
Concentration: 1 M in H2O
Impurities: DNases, none detected; RNases, none detected; Insoluble matter, passes filter test; Phosphatases, none detected; Proteases, none detected
Application(s) : Western blot (chemiluminescent): 10 µg/mL
pH: 9.5±0.5
Density: 1.01 g/mL at 20 °C