Anti-IL-33 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 31 kDa in Western Blots of specific cell lysates and tissues. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE and can be seen ~33kDa. Positive controls used are A20 cells and human lymphoid tissues.