IgG3, Kappa from human myeloma plasma, purified immunoglobulin, >90% (microfluidic capillary gel electrophoresis)

Synonyms: Human IgG3-kappa

MDL No.: MFCD00163926

Purity: >90% (microfluidic capillary gel electrophoresis)

Storage: -20C

UNSPSC Code: 12352203

General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG3 is the has a unique extended hinge region conferring greater flexibility Human myeloma IgG3, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application: The purified IgG3, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG3 was for in flow cytometry in CHO cells.

Physical form: Solution in 20 mM tris buffered saline, pH 8.0.

Analysis Note: The purity and identity are determined by immunoelectrophoresis, indirect ELISA and SDS-PAGE.