IgG1, Kappa from human myeloma plasma, purified immunoglobulin, >95% (SDS-PAGE)

Synonyms: Human IgG1-kappa

MDL No.: MFCD00163924

Purity: >95% (SDS-PAGE)

Storage: -20C

UNSPSC Code: 12352203

General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application: The purified IgG1, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, κ was injected into mice (500 μg) to assess the T cell and B cell responses and also used for flow cytometry in CHO cells.

Physical form: Solution in 0.02 M Tris buffered saline, pH 8.0.

Analysis Note: Identity is determined by immunoelectrophoresis and indirect ELISA.