IgG from human serum, reagent grade, >=95% (HPLC), buffered aqueous solution

Synonyms: Human IgG

MDL No.: MFCD00163923

Purity: >=95% (HPLC)

Storage: -20C

UNSPSC Code: 12352203

General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Human IgG is purified from normal human serum by precipitation and gel filtration techniques.

Application: Purified human IgG has been used :-. as blocking antibody for flow cytometry. as negative control in chromatin immunoprecipitation assays. to precipitate IgG in human larynx epidermoid carcinoma cells by immunoprecipitation. as a standard in enzyme-linked immunosorbent assay

Biochem/physiol Actions: Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide