Animal-free Recombinant Human M-CSF is expressed in human 293 cells and has an apparent molecular mass of 35 to 40 kDa due to glycosylation. M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survivial of blood monocytes, tissue macrophages, and their progenitor cells. This cytokine is produced in a human cell expression system with serum-free, chemically defined media.