Animal-free Recombinant Human HGF is expressed in human 293 cells and has an apparent molecular mass of 70 kDa. The molecular mass of HGF is lower compared to the reported range for this cytokine expressed in NS0 cells (70 to 80 kDa) which emphasizes the difference in post translational modifications. HGF is produced in a serum-free, chemically defined media. HGF is a mesanchymally derived protein mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spetrum of tissues and cell types.