KE00227 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIP5K1A ELISA kit is to be used to detect and quantify protein levels of endogenous PIP5K1A. The assay recognizes human/mouse/rat PIP5K1A. An antibody specific for PIP5K1A has been pre-coated onto the microwells. The PIP5K1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PIP5K1A is added to detect the captured PIP5K1A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.