KE00314 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human VEGFR2/KDR ELISA kit is to be used to detect and quantify protein levels of endogenous human VEGFR2/KDR. The assay recognizes human VEGFR2/KDR. An antibody specific for human VEGFR2/KDR has been pre-coated onto the microwells. The human VEGFR2/KDR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human VEGFR2/KDR is added to detect the captured human VEGFR2/KDR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.