KE00099 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Uteroglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Uteroglobin. The assay recognizes human Uteroglobin. An antibody specific for Uteroglobin has been pre-coated onto the microwells. The Uteroglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Uteroglobin is added to detect the captured Uteroglobin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.