KE00206 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The uPAR/PLAUR ELISA kit is to be used to detect and quantify protein levels of endogenous uPAR/PLAUR. The assay recognizes human uPAR/PLAUR. An antibody specific for uPAR/PLAUR has been pre-coated onto the microwells. The uPAR/PLAUR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for uPAR/PLAUR is added to detect the captured uPAR/PLAUR protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .