KE00296 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TNFSF15 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFSF15. The assay recognizes human TNFSF15. An antibody specific for TNFSF15 has been pre-coated onto the microwells. The TNFSF15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for TNFSF15 is added to detect the captured TNFSF15 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.