KE00109 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINE1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINE1. The assay recognizes human SERPINE1. An antibody specific for SERPINE1 has been pre-coated onto the microwells. The SERPINE1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for SERPINE1 is added to detect the captured SERPINE1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.