KE00260 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Progranulin/PCDGF ELISA kit is to be used to detect and quantify protein levels of endogenous Progranulin/PCDGF. The assay recognizes human Progranulin/PCDGF. An antibody specific for Progranulin/PCDGF has been pre-coated onto the microwells. The Progranulin/PCDGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Progranulin/PCDGF is added to detect the captured Progranulin/PCDGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.