KE00341 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PARK7/DJ-1 ELISA kit is to be used to detect and quantify protein levels of endogenous human PARK7/DJ-1. The assay recognizes human PARK7/DJ-1. An antibody specific for human PARK7/DJ-1 has been pre-coated onto the microwells. The human PARK7/DJ-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PARK7/DJ-1 is added to detect the captured human PARK7/DJ-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.