KE00244 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P53 ELISA kit is to be used to detect and quantify protein levels of endogenous P53. The assay recognizes human P53. An antibody specific for P53 has been pre-coated onto the microwells. The P53 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for P53 is added to detect the captured P53 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.