KE00155 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LGALS3BP ELISA kit is to be used to detect and quantify protein levels of endogenous LGALS3BP. The assay recognizes human LGALS3BP. An antibody specific for LGALS3BP has been pre-coated onto the microwells. The LGALS3BP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LGALS3BP is added to detect the captured human LGALS3BP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.