KE00287 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LDH-B ELISA kit is to be used to detect and quantify protein levels of endogenous LDH-B. The assay recognizes human LDH-B. An antibody specific for LDH-B has been pre-coated onto the microwells. The LDH-B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LDH-B is added to detect the captured LDH-B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.