KE00118 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6R ELISA kit is to be used to detect and quantify protein levels of endogenous IL6R. The assay recognizes human IL6R. An antibody specific for IL6R has been pre-coated onto the microwells. The IL6R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6R is added to detect the captured IL6R protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.