KE00293 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1RL1/ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1RL1/ST2. The assay recognizes human IL-1RL1/ST2. An antibody specific for IL-1RL1/ST2 has been pre-coated onto the microwells. The IL-1RL1/ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-1RL1/ST2 is added to detect the captured IL-1RL1/ST2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.