KE00273 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL-11 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-11. The assay recognizes human IL-11. An antibody specific for human IL-11 has been pre-coated onto the microwells. The human IL-11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human IL-11 is added to detect the captured human IL-11 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.